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Thursday, July 23, 2020 | History

2 edition of Study of the mouse PMCA4 gene found in the catalog.

Study of the mouse PMCA4 gene

Ge Yang

Study of the mouse PMCA4 gene

identification of a putative 5" regulatory region and analysis of splice variant expression .

by Ge Yang

  • 107 Want to read
  • 29 Currently reading

Published .
Written in English


About the Edition

Single-specific primer PCR was used to isolate a mouse-specific PMCA4 fragment, from which the entire cDNA was ultimately defined. A 5kb immediate upstream region of the PMCA4 locus was also isolated, and two putative transcriptional start sites were identified by primer extension. Promoter-luciferase reporter gene assays showed cell cycle-dependent repression in PMCA4 promoter, which was affected in part by c-Myb gene transfection. Alternative splicing at the amino and carboxy termini (sites A and C respectively) appeared to be regulated in a tissue-specific manner. Real-time RT-PCR revealed regulated expression of PMCA4-A and -C splice variants in response to cell cycle progression and depletion of intracellular Ca2+.PMCA4 is one of four members of the plasma membrane calcium ATPase family (PMCA1--4) of Ca2+ pumps, which serve to reduce intracellular Ca2+ concentrations. A splice variant, PMCA4CI, has a PDZ binding domain that also mediates protein-protein interactions with other PDZ domain-containing proteins, Over-expression of human PMCA4CI in vascular smooth cells (VSMC) of transgenic mice has been shown to increase blood pressure by decreasing the activity of neuronal nitric oxide synthase.

The Physical Object
Pagination103 leaves.
Number of Pages103
ID Numbers
Open LibraryOL19512852M
ISBN 100612955354

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Study of the mouse PMCA4 gene by Ge Yang Download PDF EPUB FB2

Search the world's most comprehensive index of full-text books. My library. Gene ID:updated on Oct Summary Other designations. plasma membrane calcium-transporting ATPase 4.

GeneRIFs: Gene References Into Functions. PMCA4 regulates the development of cardiac hypertrophy and provide proof of principle for a therapeutic approach to treat this condition. This study aimed to elucidate the role of PMCA4 in Ca 2+ homeostasis by characterizing the Ca 2+ balance, and renal and duodenal Ca 2+-related gene expression in PMCA4 knockout mice.

The daily water intake of PMCA4 knockout mice was significantly lower compared to Cited by: 7. PMCA4 Does Not Contribute to Global Calcium Removal. To study the role of PMCA4 in the heart, we used our mice with genetic ablation of the Pmca4 gene (PMCA4 −/−).

The absence of PMCA4 in total heart homogenates and cardiomyocytes was confirmed by Western blot (Fig. 1 A) and immunofluorescence (Fig. 1 B), by:   PMCA4 is expressed later in development than PMCA1 but is expressed more or less ubiquitously in adult mammalian organs.

PMCA gene transcription is controlled by c-Myb, leading to changes in the free intracellular Ca 2+ concentration ([Ca 2+] i) during the cell cycle progression of vascular smooth muscle cells (VSMC) (7,– 9).Cited by: Books at Amazon.

The Books homepage helps you explore Earth's Biggest Bookstore without ever leaving the comfort of your couch. Here you'll find current best sellers in books, new releases in books, deals in books, Kindle eBooks, Audible audiobooks, and. This study aimed to characterize the cardiac global gene expression profile in renal ischemia/reperfusion (IR) model using microarray technology.

To do that, left kidney ischemia was induced in male C57BL/6 mice for 60 minutes, followed by reperfusion (IR) for 5, 8, 15, or 20 days post ischemia (dpi). Question: You Are Interested In Cystic Fibrosis And Use A Mouse Model To Study The Gene CFTR.

(1pt) Design The Two Primer Sequences, Including Directionality, For The Following Segment Of CFTR: 5'-TGTCAATAAAATCCATACATTTGTGTGAAA-3' 2.

For example, the famous book The Kite Runner by Khaled Hosseini is based on real historical events of Afghanistan, but the story told in this book is actually a made up one. Genres of Books The above types of books can be further divided into many sub-categories and each of these sub-categories is a genre.

The completion of the human and mouse genome sequences opened an historic era in mammalian biology. One common conclusion from these projects was the determination that mammals have only ≈30, protein-encoding genes (1, 2).Yet, despite the apparent tractability of this figure (earlier estimates were much higher), to date all existing research has.

16 hours ago  A Foundational Study for Normal F8-Containing Mouse Models for the miRNA Regulation of Hemophilia A: Identification and Analysis of Mouse miRNAs that Downregulate the Murine F8 Gene by Katarzyna I.

Jankowska 1, Maitreyi Chattopadhyay 1,2, Zuben E. Sauna 3 and Chintamani D. Atreya 1,*. Mouse genetics has experienced many milestones over the last few decades, from the generation of inbred lines which consistently exhibited a coat color trait or susceptibility to tumors in the early s, up to routine gene manipulation, conditional targeting and whole genome sequencing or transcript profiling of individuals.

You are studying the regulatory DNA of a mouse gene expressed in developing heart, liver, and lung tissue. Your preliminary work has shown that heart and lung expression of this gene is controlled by a short fragment of DNA just upstream of the promoter.

The other functional role of the PMCA4/nNOS/α1-syntrophin complex has been underscored by the finding that the AV point mutation in the α1-syntrophin gene, which is located in the region responsible for the binding with PMCA4 is associated with long QT syndrome.

Long QT syndrome is a condition characterized by prolonged ventricular. The current study identified PMCA4 as a potential candidate as it appears to be almost uniquely over-expressed in MIA PaCa-2 cells similar to human PDAC tumors, making these an ideal model cell line to study the role of PMCA4 in PDAC.

Moreover, PMCA4 has an important role in cancer hallmark responses, including migration and apoptosis resistance. Abstract. Objectives: The PMCA gene family consists of 4 genes and at least 21 splice variants; among these, the Ca 2+ ATPase 4 (PMCA4) gene encodes a plasma membrane protein abundantly expressed in several tissues, including the kidney, heart, and ut of PMCA4 causes infertility due to immotile sperm in mouse models.

We therefore investigated variants in this gene. View mouse Atp2b4 Chr with: phenotypes, sequences, polymorphisms, proteins, references, function, expression. The laboratory mouse or lab mouse is a small mammal of the order Rodentia which is bred and used for scientific tory mice are usually of the species Mus are the most commonly used mammalian research model and are used for research in genetics, psychology, medicine and other scientific disciplines.

Mice belong to the Euarchontoglires. Gene knockout of either EPO or EpoR in mice is embryonic lethal at around embryonic day (E) (Lin et al., ; Wu et al., ), but the expression of an erythroid-specific EpoR transgene under the control of GATA-1 hematopoietic regulatory domain rescues the lethal phenotype of the EpoR −/− mice (Suzuki et al., ).These mice (ΔEpoR E) show no gross.

Zebrafish (Danio rerio), largely used as a model for studying developmental processes, has also emerged as a valuable system for modelling human inflammatory diseases.

However, in. Transcriptional bursting is a major source of noise in gene expression. The telegraph model of gene expression, whereby transcription switches between on and off states, is the dominant model for bursting. Recently it was shown that the telegraph model cannot explain a number of experimental observations from perturbation data.How can you generate a mouse that expresses a gene on demand as an adult mouse.

none of the above using a tissue specific promotor to drive expression of a tetracycline response element and ubiquitous promotor to control expression of the gene of interest with lots of training using a tissue specific promotor to drive expression of a tetracycline reregulatory protein and a .The KLHL4 gene, localized on the X chromosome, associates with a disorder known as X-linked cleft palate (CPX).

However, the biological functions of KLHL4 are largely unknown. In this study, microarray analysis of HEKA embryonic kidney cells, expressing ectopic p53, showed a 3-fold increase of KLHL4 mRNA.